Molecular analysis of markers associated with chloroquine and sulfadoxine/pyrimethamine resistance in Plasmodium falciparum malaria parasites from southeastern Côte-d'Ivoire by the time of Artemisinin-based Combination Therapy adoption in 2005.
Identifieur interne : 001289 ( Main/Exploration ); précédent : 001288; suivant : 001290Molecular analysis of markers associated with chloroquine and sulfadoxine/pyrimethamine resistance in Plasmodium falciparum malaria parasites from southeastern Côte-d'Ivoire by the time of Artemisinin-based Combination Therapy adoption in 2005.
Auteurs : Berenger Aristide Ako [Côte d'Ivoire] ; André Toure Offianan [Côte d'Ivoire] ; Marnie Johansson [États-Unis] ; Louis Koné Penali [Côte d'Ivoire] ; Simon-Pierre Assanvo Nguetta [Côte d'Ivoire] ; Carol Hopkin Sibley [États-Unis]Source :
- Infection and Drug Resistance [ 1178-6973 ] ; 2012.
Descripteurs français
- mix :
Abstract
PURPOSE: Artemisin-based combination therapies became the recommended therapy in Côte-d'Ivoire in 2005, but both chloroquine (CQ) and sulfadoxine/pyrimethamine (SP) have been heavily used for many decades. Despite this long history, little is known about the geographical distribution of drug resistance-conferring genotypes outside the capital city of Abidjan. In this work, we compared the prevalence of drug-resistant genotypes in Bonoua, an urban area, and Samo, a rural agricultural area, in southeastern Côte-d'Ivoire, about 59 km from Abidjan. PATIENTS AND METHODS: Samples were collected from symptomatic patients in both sites during the rainy season in 2005. Genomic DNA was isolated and codons associated with resistance to CQ and SP were analyzed: pfcrt codons Cys-72-Ser, Val-73-Val, Met-74-Ile, Arg-75-Glu, Lys-76-Thr; pfdhfr codons Ala-16-Val, Arg-51-Ile, Cys-59-Arg, Ser-108-Arg/Thr, and Ile-164-Leu; pfdhps codons Ser-436-Ala, Ala-437-Gly, Lys-540-Glu, Ala-581-Gly, and Ala-613-Thr/Ser. RESULTS: A limited number of genotypes were found in Bonoua compared with Samo. In both sites, the triple-mutant allele CVIET of pfcrt predominated: 100% in Bonoua and 86.2% in Samo. The wild-type allele, NCSI of pfdhfr, was common - 50% in Bonoua and 38.7% in Samo - but the triple-mutant IRNI and double-mutant NRNI were also frequent (IRNI, 32.6% in Bonoua and 19.4% in Samo; NRNI, 15.2% in Bonoua and 9.7% in Samo). In Samo, a wide range of different genotypes of Pfdhps was observed, with alleles carrying the Gly-437 codon fixed in Bonoua and comprising 73% of the isolates in Samo. CONCLUSION: Although these two sites are only 8 km apart, they belonged to very different ecological environments. The overall prevalence of alleles of single-nucleotide polymorphisms associated with resistance to CQ and SP in both locations was among the highest of the region by 2005, although the more rural site showed a more diverse set of alleles and mixed infections. Continued surveillance of these markers will be a useful tool for drug policy, as both CQ and SP are still frequently used years after withdrawal, and SP is recommended by the World Health Organization for intermittent preventive therapy for pregnant women and infants. Data analyzed herein are among the first to be generated during the year of artemisin-based combination-therapy introduction in Côte-d'Ivoire and could be of some interest for malaria policy-makers.
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DOI: 10.2147/IDR.S31409
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<addrLine>1705 NE Pacific St #K-357 / Box 357730 Seattle WA 98195-7730</addrLine> <country key="US"></country> </address> <ref type="url">http://gs.washington.edu/</ref> </desc> <listRelation> <relation active="#struct-300433" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-300433" type="direct"><org type="institution" xml:id="struct-300433" status="VALID"> <orgName>University of Washington [Seattle]</orgName> <desc> <address> <addrLine>Seattle, Washington 98105</addrLine> <country key="US"></country> </address> <ref type="url">http://www.washington.edu/</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>États-Unis</country></affiliation></author></analytic><idno type="DOI">10.2147/IDR.S31409</idno><series><title level="j">Infection and Drug Resistance</title><idno type="ISSN">1178-6973</idno><imprint><date type="datePub">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="mix" xml:lang="fr"><term>Côte-d'Ivoire</term><term>chloroquine</term><term>malaria resistance</term><term>pfcrt</term><term>pfdhfr</term><term>pfdhps</term><term>sulfadoxine/pyrimethamine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"> <p>PURPOSE: Artemisin-based combination therapies became the recommended therapy in Côte-d'Ivoire in 2005, but both chloroquine (CQ) and sulfadoxine/pyrimethamine (SP) have been heavily used for many decades. Despite this long history, little is known about the geographical distribution of drug resistance-conferring genotypes outside the capital city of Abidjan. In this work, we compared the prevalence of drug-resistant genotypes in Bonoua, an urban area, and Samo, a rural agricultural area, in southeastern Côte-d'Ivoire, about 59 km from Abidjan. PATIENTS AND METHODS: Samples were collected from symptomatic patients in both sites during the rainy season in 2005. Genomic DNA was isolated and codons associated with resistance to CQ and SP were analyzed: pfcrt codons Cys-72-Ser, Val-73-Val, Met-74-Ile, Arg-75-Glu, Lys-76-Thr; pfdhfr codons Ala-16-Val, Arg-51-Ile, Cys-59-Arg, Ser-108-Arg/Thr, and Ile-164-Leu; pfdhps codons Ser-436-Ala, Ala-437-Gly, Lys-540-Glu, Ala-581-Gly, and Ala-613-Thr/Ser. RESULTS: A limited number of genotypes were found in Bonoua compared with Samo. In both sites, the triple-mutant allele CVIET of pfcrt predominated: 100% in Bonoua and 86.2% in Samo. The wild-type allele, NCSI of pfdhfr, was common - 50% in Bonoua and 38.7% in Samo - but the triple-mutant IRNI and double-mutant NRNI were also frequent (IRNI, 32.6% in Bonoua and 19.4% in Samo; NRNI, 15.2% in Bonoua and 9.7% in Samo). In Samo, a wide range of different genotypes of Pfdhps was observed, with alleles carrying the Gly-437 codon fixed in Bonoua and comprising 73% of the isolates in Samo. CONCLUSION: Although these two sites are only 8 km apart, they belonged to very different ecological environments. The overall prevalence of alleles of single-nucleotide polymorphisms associated with resistance to CQ and SP in both locations was among the highest of the region by 2005, although the more rural site showed a more diverse set of alleles and mixed infections. Continued surveillance of these markers will be a useful tool for drug policy, as both CQ and SP are still frequently used years after withdrawal, and SP is recommended by the World Health Organization for intermittent preventive therapy for pregnant women and infants. Data analyzed herein are among the first to be generated during the year of artemisin-based combination-therapy introduction in Côte-d'Ivoire and could be of some interest for malaria policy-makers.</p> </div></front></TEI><affiliations><list><country><li>Côte d'Ivoire</li><li>États-Unis</li></country></list><tree><country name="Côte d'Ivoire"><noRegion><name sortKey="Ako, Berenger Aristide" sort="Ako, Berenger Aristide" uniqKey="Ako B" first="Berenger Aristide" last="Ako">Berenger Aristide Ako</name></noRegion><name sortKey="Nguetta, Simon Pierre Assanvo" sort="Nguetta, Simon Pierre Assanvo" uniqKey="Nguetta S" first="Simon-Pierre Assanvo" last="Nguetta">Simon-Pierre Assanvo Nguetta</name><name sortKey="Offianan, Andre Toure" sort="Offianan, Andre Toure" uniqKey="Offianan A" first="André Toure" last="Offianan">André Toure Offianan</name><name sortKey="Penali, Louis Kone" sort="Penali, Louis Kone" uniqKey="Penali L" first="Louis Koné" last="Penali">Louis Koné Penali</name></country><country name="États-Unis"><noRegion><name sortKey="Johansson, Marnie" sort="Johansson, Marnie" uniqKey="Johansson M" first="Marnie" last="Johansson">Marnie Johansson</name></noRegion><name sortKey="Sibley, Carol Hopkin" sort="Sibley, Carol Hopkin" uniqKey="Sibley C" first="Carol Hopkin" last="Sibley">Carol Hopkin Sibley</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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